Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Smarca4

Cell type

Cell type Class
Blood
Cell type
Acute myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
MN1-driven leukemia
cell type
AML
chip antibody
Smarca4

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HSPCs were isolated and transduced with the respective plasmid, then sorted. After 7-9 days in culture ChIP was performed. Briefly, samples were crosslinked, lysed, sonicated and immunoprecipated with the indicated antibody overnight. After a series of washes they were reverse crosslinked overnight and DNA extracted. Library prep was performed at Genewiz, Illumina HiSeq, 2x150bp paired-end reads, multiplexed

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
29420445
Reads aligned (%)
83.8
Duplicates removed (%)
15.8
Number of peaks
17889 (qval < 1E-05)

mm9

Number of total reads
29420445
Reads aligned (%)
83.7
Duplicates removed (%)
15.7
Number of peaks
17832 (qval < 1E-05)

Base call quality data from DBCLS SRA